MPs have rejected the government’s request for a three-day recess while the Conservatives stage their annual party conference.
It is the seventh vote the government has lost out of eight held in the Commons since Boris Johnson became PM.
MPs voted 306 to 289 to reject the motion asking for the Commons to adjourn until Thursday next week.
It follows Parliament resuming on Wednesday, after the Supreme Court ruled its suspension was unlawful.
The Conservative Party conference is due to be held in Manchester from Sunday to Wednesday.
Labour and the Liberal Democrats have already held their annual party conferences.
Following the MPs’ vote, Commons Leader Jacob Rees-Mogg announced the business for the House when it sits next week, including a debate on the Domestic Abuse Bill, which has cross-party support, on Wednesday.
Prime Minister Boris Johnson is due to give his keynote speech at the Conservative Party conference on Wednesday, but that will now clash with Prime Minister’s Questions in the Commons.
Mr Rees-Mogg told MPs he assumed the prime minister would “make his normal appearance” in the Commons.
Liberal Democrat Brexit spokesman Tom Brake said he was “pleased” MPs had voted against a conference recess.
“We have only just resumed sitting after being unlawfully dismissed by Boris Johnson,” he tweeted. “Now was not the time to grant the government additional time to sit on its hands and avoid scrutiny.”
Mr Johnson has been urged to apologise after he said the best way to honour Jo Cox, the Labour MP murdered during the 2016 EU referendum campaign, was to get “Brexit done” in the Commons on Wednesday.
He was also criticised for calling the law aimed at blocking a no-deal Brexit the “surrender bill”.
Independent MP Nick Boles, formerly a Conservative, said he had planned to abstain on the recess vote but decided to oppose the government, tweeting: “After the PM’s behaviour yesterday I think it is crucial that he be held to account at PMQs next Wednesday.”
Parliament: MPs reject recess for Tory conference}